Leff J, Krinsky N I
Science. 1967 Dec 8;158(3806):1332-4. doi: 10.1126/science.158.3806.1332.
Mutant cells lackng chlorophyll, chloroplasts, and chloroplast DNA were produced by irradiating Euglena gracilis in aerobic conditions with visible or red light (greater than 610 nanometers) of an intensity equivalent to that of direct sunlight. The photosensitizer is apparently the endogenous chlorophyll present in the chloroplasts. These mutants are comparable to those induced by ultraviolet light, x-rays, heat, or streptomycin. Our findings indicate that visible light can serve as a mutagenic agent in the absence of exogenous photosensitizers, thus directly effecting the course of evolution of organisms containing chlorophyll.
通过在有氧条件下，用强度等同于直射阳光的可见光或红光（波长大于610纳米）照射纤细裸藻，产生了缺乏叶绿素、叶绿体和叶绿体DNA的突变细胞。光敏剂显然是叶绿体中存在的内源性叶绿素。这些突变体与紫外线、X射线、热或链霉素诱导产生的突变体相似。我们的研究结果表明，在没有外源性光敏剂的情况下，可见光可以作为诱变剂，从而直接影响含叶绿素生物体的进化过程。
