含硫醇嘌呤和核糖核酸的亲和层析
Affinity chromatography of thiol-containing purines and ribonucleic acid.
作者信息
Melvin W T, Keir H M
出版信息
Biochem J. 1977 Dec 15;168(3):595-7. doi: 10.1042/bj1680595.
Abstract
Evidence is presented for the specific interaction of 6-mercaptopurine with mercurated cellulose. Following from this, a new method is described for the affinity chromatography of thiol-containing molecules and of RNA containing incorporated 6-thioguanosine on columns of mercurated cellulose. This technique may find application in the study of RNA metabolism and gene expression.
摘要
有证据表明6-巯基嘌呤与汞化纤维素存在特异性相互作用。据此，描述了一种新方法，用于在汞化纤维素柱上对含硫醇分子以及含有掺入的6-硫代鸟苷的RNA进行亲和层析。该技术可能在RNA代谢和基因表达研究中得到应用。
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