Dugal R, Dupuis C, Bertrand M J
Br J Sports Med. 1977 Dec;11(4):162-9. doi: 10.1136/bjsm.11.4.162.
Recently developed radioimmunoassays (RIA) for the analysis of anabolic steroids and their metabolites in biological fluids were tested for cross-reactivity with other types of steroids. Results show that the degree of desirable cross-reactivity within the two classes or orally active anabolic steroids vary widely and that the antiserum for 19-Nortestosterone (the active principle of intramuscular preparations) has a very high degree of undesirable cross-reactivity with components of oral contraceptives. Single and multiple dose studies in human volunteers demonstrate that the detection level and degree of retrospectivity are likewise variable but that the test easily detects most anabolic steroids during treatment. At the present time, the combination of the three antisera for the assay of a sample appears to be a relatively rapid and economic method for screening large numbers of samples in situations where doping control of anabolic steroids is required. The importance of utilizing physico-chemical means for identification of RIA potential positives is emphasized.
最近开发的用于分析生物体液中合成代谢类固醇及其代谢物的放射免疫分析（RIA）方法，针对与其他类型类固醇的交叉反应性进行了测试。结果表明，两类口服活性合成代谢类固醇内所需的交叉反应程度差异很大，且19-去甲睾酮（肌肉注射制剂的活性成分）的抗血清与口服避孕药的成分具有非常高的不良交叉反应程度。在人类志愿者中进行的单剂量和多剂量研究表明，检测水平和追溯程度同样存在差异，但该测试在治疗期间能轻松检测出大多数合成代谢类固醇。目前，在需要对合成代谢类固醇进行兴奋剂检测的情况下，使用三种抗血清组合检测样本似乎是一种相对快速且经济的方法，可用于筛查大量样本。文中强调了利用物理化学方法鉴定放射免疫分析潜在阳性结果的重要性。
