Morozov V I, Chaĭkovskiĭ V S
Vopr Med Khim. 1985 Jul-Aug;31(4):131-5.
Characteristic properties of radioimmunoassay (RIA) of steroids with 3H- and 124I-ligands as a label were studied using two anabolic steroids methandrostenolone and 19-nortestosterone. Antisera towards 124I-steroids were used at higher dilutions as their specific activity was distinctly more than in the antisera to 3H-substances. However, 125I-steroid must be in a stereochemical correspondence with the antiserum otherwise antibodies do not bind the label. The antisera towards 3H-steroids were obtained after coupling of a carrier at any position of the steroid molecule. Standard curves for 3H-steroids were very steep; this pattern of the curves enabled to increase the accuracy of calculations. Sensitivity of RIA with 3H-label was also higher as compared with 125I-steroids in the experiments where unlabelled steroids inhibited the label binding with antibodies by 50%. Distinct differences were not observed in RIA specificity for methandrostenolone and 19-nortestosterone.
以3H和124I配体作为标记，使用两种合成代谢类固醇美雄酮和19-去甲睾酮研究了类固醇放射免疫分析（RIA）的特性。针对124I-类固醇的抗血清在更高稀释度下使用，因为它们的比活性明显高于针对3H物质的抗血清。然而，125I-类固醇必须与抗血清具有立体化学对应关系，否则抗体不会结合标记物。在类固醇分子的任何位置偶联载体后获得针对3H-类固醇的抗血清。3H-类固醇的标准曲线非常陡峭；这种曲线模式能够提高计算的准确性。在未标记类固醇将标记物与抗体的结合抑制50%的实验中，与125I-类固醇相比，3H标记的RIA灵敏度也更高。在对美雄酮和19-去甲睾酮的RIA特异性方面未观察到明显差异。
