Hokin M R
J Gen Physiol. 1967 Oct;50(9):2197-209. doi: 10.1085/jgp.50.9.2197.
In goose salt gland slices incubated in bicarbonate-buffered medium which contained 170 mEq of Na(+)/liter, net total tissue Na(+), expressed as milliequivalents per kilogram, was, in the presence of either acetylcholine (plus eserine) or ouabain, significantly higher than that of the bathing fluid. Acetylcholine caused an increase in the tissue Na(+) content as compared with untreated slices; there was an approximately equivalent decrease in K(+) and a significant decrease in Cl(-). The calculated net intracellular concentrations of Na(+), expressed as milliequivalents per liter of intracellular water, in unstimulated, acetylcholine-stimulated, and ouabain-treated slices were 2.1, 3.1, and 2.7 times higher, respectively, than the concentration of Na(+) in the bathing fluid. The net intracellular concentration of Na(+), expressed as milliequivalents per liter of intracellular water, in slices incubated in the presence of acetylcholine was 531 mEq/liter; this is approximately the same as the concentration of Na(+) in the secreted fluid of the goose salt gland (515 mEq/liter). The results indicate that the main concentration gradient for Na(+) could be established across the basal membrane. The data do not indicate whether this involves active transport of Na(+) per se. A second stage which might involve Na-K ATPase activity at the luminal membrane is discussed. The sum of the total tissue Na(+) and K(+) was approximately 250 mEq/kg, whereas the Cl(-) content was only approximately 130 mEq/kg.
在含有170mEq/L Na⁺的碳酸氢盐缓冲培养基中孵育的鹅盐腺切片，在乙酰胆碱（加毒扁豆碱）或哇巴因存在的情况下，以每千克毫当量表示的组织总Na⁺净含量显著高于浴液中的含量。与未处理的切片相比，乙酰胆碱使组织Na⁺含量增加；K⁺含量大约等量减少，Cl⁻含量显著减少。以每升细胞内水的毫当量表示的未刺激、乙酰胆碱刺激和哇巴因处理的切片中Na⁺的计算净细胞内浓度分别比浴液中Na⁺浓度高2.1倍、3.1倍和2.7倍。在乙酰胆碱存在下孵育的切片中，以每升细胞内水的毫当量表示的Na⁺净细胞内浓度为531mEq/L；这与鹅盐腺分泌液中Na⁺的浓度（515mEq/L）大致相同。结果表明，Na⁺的主要浓度梯度可以跨基底膜建立。数据未表明这是否涉及Na⁺本身的主动转运。讨论了可能涉及腔膜上Na⁺-K⁺ ATP酶活性的第二阶段。组织总Na⁺和K⁺的总和约为250mEq/kg，而Cl⁻含量仅约为130mEq/kg。
