用于检测对结核菌素纯蛋白衍生物（PPD）和肾癌抗原的细胞介导超敏反应的琼脂糖微滴白细胞迁移技术。
Agarose microdroplet leucocyte migration technique for detection of cell-mediated hypersensitivity to PPD and renal carcinoma antigen.
作者信息
Sorensen T B, Kjaer M
出版信息
Clin Exp Immunol. 1977 Oct;30(1):168-72.
Abstract
The agarose microdroplet leucocyte migration technique is an in vitro technique for detection of cell-mediated hypersensitivity in man, using only 10-15% of the volume of blood required for other leucocyte migration techniques. The technique is described in detail and results are presented from migrations with leucocytes from hypernephroma patients and controls tested with hypernephroma extract and normal kidney extract, and from tuberculin-positive and tuberculin-negative persons tested with PPD.
摘要
琼脂糖微滴白细胞迁移技术是一种用于检测人体细胞介导超敏反应的体外技术，与其他白细胞迁移技术相比，该技术所需血量仅为其10 - 15%。本文详细描述了该技术，并展示了用肾细胞癌提取物和正常肾提取物检测肾细胞癌患者及对照白细胞迁移的结果，以及用结核菌素纯蛋白衍生物检测结核菌素阳性和阴性者白细胞迁移的结果。
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The agarose migration inhibition technique for in vitro demonstration of cell-mediated immunity in man. A review.用于体外证明人体细胞介导免疫的琼脂糖迁移抑制技术。综述。
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Comparison between the direct capillary tube leucocyte migration technique (LMCT) and the direct agarose leucocyte migration technique (LMAT) as indicators of tumour-directed cell-mediated hypersensitivity (TCMH) in patients with renal carcinoma.直接毛细管白细胞迁移技术（LMCT）与直接琼脂糖白细胞迁移技术（LMAT）作为肾癌患者肿瘤定向细胞介导超敏反应（TCMH）指标的比较。
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Technical study of the leucocyte migration inhibition test in agarose. Application to PPD and to hepatitis B antigen.琼脂糖中白细胞迁移抑制试验的技术研究。在结核菌素纯蛋白衍生物及乙肝抗原中的应用。
Clin Exp Immunol. 1974 Nov;18(3):439-48.
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The capillary tube leucocyte migration technique (LMT) as a method for detection of serum inhibiting activity of tumour-directed, cellular hypersensitivity in patients with renal carcinomas.毛细管白细胞迁移技术（LMT）作为检测肾癌患者肿瘤定向细胞超敏反应血清抑制活性的一种方法。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Apr;82(2):294-6.
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Human cell-mediated immunity to tuberculin as assayed by the agarose micro-droplet leukocyte migration inhibition technique: comparison with the capillary tube assay.采用琼脂糖微滴白细胞迁移抑制技术测定人对结核菌素的细胞介导免疫：与毛细管试验的比较。
J Immunol Methods. 1977;15(4):355-71. doi: 10.1016/0022-1759(77)90097-7.
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Checking of carcinoma patients with the leukocyte migration technique (LMT) under agarose.采用琼脂糖下白细胞迁移技术（LMT）对癌症患者进行检查。
Neoplasma. 1978;25(4):483-91.
本文引用的文献
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Tuberculin-induced migration inhibition of human peripheral leucocytes in agarose medium.结核菌素诱导的人外周血白细胞在琼脂糖培养基中的游走抑制
Acta Allergol. 1971 Feb;26(1):56-80. doi: 10.1111/j.1398-9995.1971.tb01399.x.
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A micro-method for peripheral leucocyte migration in tuberculin sensitivity.结核菌素敏感性外周白细胞迁移的微量法。
J Clin Pathol. 1971 Sep;24(6):533-6. doi: 10.1136/jcp.24.6.533.
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The dose-related effect of tumour extract on the in vitro migration of leucocytes from patients with renal carcinoma.肿瘤提取物对肾癌患者白细胞体外迁移的剂量相关效应。
Eur J Cancer (1965). 1975 Apr;11(4):281-9. doi: 10.1016/0014-2964(75)90009-2.
5
Prognostic value of tumour-directed, cell-mediated hypersensitivity detected by means of the leucocyte migration technique in patients with renal carcinoma.通过白细胞迁移技术检测的肿瘤定向细胞介导超敏反应在肾癌患者中的预后价值。
Eur J Cancer (1965). 1976 Nov;12(11):889-98. doi: 10.1016/0014-2964(76)90006-2.
6
Comparison between the direct capillary tube leucocyte migration technique (LMCT) and the direct agarose leucocyte migration technique (LMAT) as indicators of tumour-directed cell-mediated hypersensitivity (TCMH) in patients with renal carcinoma.直接毛细管白细胞迁移技术（LMCT）与直接琼脂糖白细胞迁移技术（LMAT）作为肾癌患者肿瘤定向细胞介导超敏反应（TCMH）指标的比较。
Acta Allergol. 1976 Apr;31(2):141-58. doi: 10.1111/j.1398-9995.1976.tb01488.x.
7
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