Verbin R S, Farber E
J Cell Biol. 1967 Dec;35(3):649-58. doi: 10.1083/jcb.35.3.649.
A single injection of 1.5 mg/kg of cycloheximide induces a complete disappearance of mitotic activity in rat intestinal crypts within 1.5-2 hr. No significant necrosis of crypt cells is observed even though this phenomenon is accompanied by a marked decrease in uptake of labeled precursors into protein and DNA. Mitoses reappear 6 hr after injection and recovery then follows a cyclic pattern over a period equivalent to one cell cycle, thereby reflecting at least a partial synchronization of cell division. Concurrent use of colchicine, an agent known to induce metaphase arrest, has demonstrated that cycloheximide, while having no apparent effect on cells already in division, prevents the entrance of new cells into visible mitosis. Analysis of the cell cycle suggests that one block initiated by cycloheximide occurs in G(2), presumably as the result of an interference with the formation of protein(s) required for the normal progression of cells from this phase of the cycle into mitosis.
单次注射1.5毫克/千克的放线菌酮会在1.5至2小时内使大鼠肠隐窝中的有丝分裂活性完全消失。尽管这一现象伴随着标记前体进入蛋白质和DNA的摄取显著减少，但未观察到隐窝细胞有明显坏死。注射后6小时有丝分裂重新出现，随后在相当于一个细胞周期的时间段内恢复呈周期性模式，从而至少反映了细胞分裂的部分同步化。同时使用已知能诱导中期停滞的秋水仙碱表明，放线菌酮虽然对已经在分裂的细胞没有明显影响，但会阻止新细胞进入可见的有丝分裂。细胞周期分析表明，放线菌酮引发的一个阻滞发生在G(2)期，推测是由于干扰了细胞从周期的这一阶段正常进入有丝分裂所需蛋白质的形成。
