Mackenzie C D, Ramalho-Pinto F J, McLaren D J, Smithers S R
Clin Exp Immunol. 1977 Oct;30(1):97-104.
Eosinophils from the peritoneal washings of normal rats adhered to live or formalin-fixed schistosomula of Schistosoma mansoni in vitro, in the presence of heat-inactivated serum from infected rats. Eosinophil adherence caused permeability changes in the schistosomula as revealed by 51Cr release and methylene blue uptake. The serum factor which mediated adherence resided in the 7S fraction after Sephadex G-200 gel filtration. Protein A from Staphylococcus aureus which binds specifically to the Fc piece of IgG inhibited adherence, thereby demonstrating that IgG was the antibody responsible for this reaction and that the Fc portion was the site of interaction between eosinophil and antibody; rat eosinophils were shown to possess Fc receptors. The antibody mediating adherence reached high titres in the sera of rats 5-8 weeks after exposure to 500 cercariae, but thereafter there was a gradual decline in titre. Surface membrane from adult S. mansoni inhibited adherence, indicating the presence of cross-reacting antigens in adult worms and schistosomula.
在存在感染大鼠的热灭活血清的情况下，正常大鼠腹腔洗液中的嗜酸性粒细胞在体外可黏附于曼氏血吸虫的活虫体或福尔马林固定的童虫。如通过51Cr释放和亚甲蓝摄取所显示的，嗜酸性粒细胞黏附导致童虫通透性改变。经葡聚糖凝胶G-200凝胶过滤后，介导黏附的血清因子存在于7S组分中。金黄色葡萄球菌的蛋白A可特异性结合IgG的Fc片段，它抑制了黏附，从而证明IgG是负责此反应的抗体，且Fc部分是嗜酸性粒细胞与抗体之间的相互作用位点；已表明大鼠嗜酸性粒细胞具有Fc受体。介导黏附的抗体在接触500条尾蚴后5至8周的大鼠血清中达到高滴度，但此后滴度逐渐下降。曼氏血吸虫成虫的表面膜抑制黏附，表明成虫和童虫中存在交叉反应抗原。
