Moser G, Sher A
J Immunol. 1981 Mar;126(3):1025-9.
Purified human eosinophils and neutrophils were compared for their capacity to mediate anti-TNP antibody-dependent killing of schistosomula of Schistosoma mansoni surface labeled with TNBS. In contrast with findings from studies employing human anti-schistosome sera and unmodified larvae, neutrophils in addition to eosinophils were found to be potent killers of schistosomula. Indeed, neutrophils proved to be consistently more effective than eosinophils in killing haptenated larvae throughout the dose response of each of the components (i.e., the TNBS surface label, rabbit anti-TNP sera and cells) of the in vitro reaction. The capacity of neutrophils to kill haptenated parasites is not likely to be due to the use of rabbit antibodies in the assays, since these cells were ineffective in killing unlabeled schistosomula when either rabbit or human anti-schistosome sera were employed. Furthermore, the ability of neutrophils to destroy TNP-labeled schistosomula does not appear to result from sublethal damage induced by the labeling procedure, since haptenated parasites were found to be no more susceptible than unmodified worms to killing mediated by anti-schistosome sera plus eosinophils or complement and, like unlabeled parasites, could not be killed by anti-schistosome sera plus neutrophils. The lethal interaction of neutrophils and haptenated schistosomula was preceded by a rapid and marked adherence of the cells to the worms, an effect that was not observed in the nonlethal interaction of neutrophils with schistosomula induced by anti-schistosome sera. The results argue, therefore, that the capacity of different granulocyte populations to kill schistosomula may be determined by the kinetics and intensity with which the effector cells adhere to the parasites. The findings further suggest that a key factor influencing the differential adherence of the cells to the parasites and their subsequent death may be the nature of the specific antigen-antibody interaction occurring at the surface of the larvae.
对纯化的人嗜酸性粒细胞和中性粒细胞介导抗三硝基苯（TNP）抗体依赖性杀伤用三硝基苯磺酸（TNBS）表面标记的曼氏血吸虫童虫的能力进行了比较。与使用人抗血吸虫血清和未修饰幼虫的研究结果相反，发现除嗜酸性粒细胞外，中性粒细胞也是童虫的有效杀伤者。实际上，在体外反应的每个组分（即TNBS表面标记、兔抗TNP血清和细胞）的剂量反应过程中，中性粒细胞在杀伤半抗原化幼虫方面始终比嗜酸性粒细胞更有效。中性粒细胞杀伤半抗原化寄生虫的能力不太可能归因于试验中使用兔抗体，因为当使用兔或人抗血吸虫血清时，这些细胞在杀伤未标记的童虫方面无效。此外，中性粒细胞破坏TNP标记童虫的能力似乎不是由标记过程诱导的亚致死损伤导致的，因为发现半抗原化寄生虫与未修饰的虫体相比，对抗血吸虫血清加嗜酸性粒细胞或补体介导的杀伤并不更敏感，并且与未标记的寄生虫一样，不能被抗血吸虫血清加中性粒细胞杀死。中性粒细胞与半抗原化童虫的致死性相互作用之前，细胞会迅速且显著地黏附于虫体，而在抗血吸虫血清诱导的中性粒细胞与童虫的非致死性相互作用中未观察到这种效应。因此，这些结果表明，不同粒细胞群体杀伤童虫的能力可能由效应细胞黏附于寄生虫的动力学和强度决定。这些发现进一步表明，影响细胞对寄生虫的差异黏附及其随后死亡的一个关键因素可能是幼虫表面发生的特异性抗原 - 抗体相互作用的性质。
