用噬菌体φ-X174免疫的豚鼠中免疫球蛋白的生物合成位点。
Sites of biosynthesis of immunoglobulins in guinea-pigs immunized with bacteriophage phi-X174.
作者信息
Fleming W A, Wilkinson P C, White R G
出版信息
Immunology. 1967 Dec;13(6):613-22.
The sites of biosynthesis of 19S and 7S antibody in the guinea-pig were determined by assay of the phage neutralizing activity in cultures of lymph node, bone marrow and splenic tissues from guinea-pigs immunized with bacteriophage ΦX174. Per unit weight of tissue, much higher levels of 19S antibody were synthesized by bone marrow tissue cultures than by those of the spleen or the lymph nodes draining the site of antigen injection. On the other hand, the levels of synthesis of 7S antibody per unit weight of tissue were higher in cultures of the homolateral and contralateral lymph nodes draining the site of injection into the footpad than in cultures of spleen or bone marrow. Evidence was presented that the phage neutralizing activity observed was, in fact, due to synthesis of specific antibody.
通过检测用噬菌体ΦX174免疫的豚鼠的淋巴结、骨髓和脾脏组织培养物中的噬菌体中和活性，确定了豚鼠体内19S和7S抗体的生物合成部位。每单位组织重量，骨髓组织培养物合成的19S抗体水平比脾脏或引流抗原注射部位的淋巴结高得多。另一方面，每单位组织重量的7S抗体合成水平，在足垫注射部位同侧和对侧引流淋巴结的培养物中高于脾脏或骨髓培养物。有证据表明，观察到的噬菌体中和活性实际上是由于特异性抗体的合成。
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