佐剂对豚鼠体内针对噬菌体phiX174的19S和7S抗体生物合成的影响。
The effect of adjuvants on biosynthesis of 19S and 7S antibody against bacteriophage phiX174 in the guinea-pig.
作者信息
Wilkinson P C, Fleming W A, White R G
出版信息
Immunology. 1967 Dec;13(6):603-11.
Guinea-pigs given a single injection of bacteriophage ΦX174 produced 19S antibody 8 days later. The levels of 19S antibody attained were not significantly different in animals injected with bacteriophage in water-in-oil emulsions with and without added mycobacteria, in bentonite or in saline alone. On the other hand, while both 7S γ- and γ-immunoglobulin antibodies were present 21 days after a single injection of bacteriophage ΦX174 in saline alone, the addition of water-in-oil emulsions to the injection mixture led to enhanced levels of 7S antibody and the addition of mycobacteria to these emulsions increased 7S antibody levels still further. Delayed hypersensitivity reactions were detected by corneal testing in a proportion of the guinea-pigs injected with bacteriophage in water-in-oil emulsions whether or not mycobacteria were also present in the mixture. Guinea-pigs immunized with bacteriophage in saline showed negative corneal tests.
单次注射噬菌体ΦX174的豚鼠在8天后产生了19S抗体。在注射含或不含添加分枝杆菌的油包水乳剂中的噬菌体、膨润土中的噬菌体或仅注射生理盐水的动物中，所达到的19S抗体水平没有显著差异。另一方面，虽然在仅单次注射生理盐水的噬菌体ΦX174 21天后，同时存在7Sγ和γ免疫球蛋白抗体，但在注射混合物中添加油包水乳剂会导致7S抗体水平升高，而在这些乳剂中添加分枝杆菌会使7S抗体水平进一步提高。通过角膜试验在一部分注射了含或不含分枝杆菌的油包水乳剂中的噬菌体的豚鼠中检测到了迟发型超敏反应。用生理盐水免疫噬菌体的豚鼠角膜试验呈阴性。
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