豚鼠乳腺中3-磷酸甘油的酯化作用。
Esterification of glycerol 3-phosphate in lactating guinea-pig mammary gland.
作者信息
Kuhn N J
出版信息
Biochem J. 1967 Oct;105(1):213-23. doi: 10.1042/bj1050213.
Abstract
- The presence of palmitoyl-CoA-l-glycerol 3-phosphate palmitoyltransferase (EC 2.3.1.15) has been demonstrated in a particulate fraction of mammary tissue from lactating guinea pigs. 2. Cell-free preparations also catalysed the activation of palmitate and oleate, and the conversion of enzymically formed phosphatidic acid into glycerides, in accord with the Kennedy pathway of glyceride formation. 3. The properties of the system that esterifies l-glycerol 3-phosphate were studied with respect to substrates and cofactors, and the reaction product was shown to be phosphatidic acid (1,2-diacyl glycerol 3-phosphate). 4. The extent to which newly formed phosphatidic acid was converted into glyceride in a cell-free system was dependent on the nature of the acyl donor, the concentration of subcellular particles, the time of incubation and the concentration of Mg(2+).
摘要
- 已在泌乳豚鼠乳腺组织的微粒体部分证实存在棕榈酰辅酶A - L - 甘油3 - 磷酸棕榈酰转移酶（EC 2.3.1.15）。2. 无细胞制剂也催化了棕榈酸和油酸的活化，以及酶促形成的磷脂酸向甘油酯的转化，这与甘油酯形成的肯尼迪途径一致。3. 针对底物和辅因子研究了酯化L - 甘油3 - 磷酸的系统特性，反应产物显示为磷脂酸（1,2 - 二酰基甘油3 - 磷酸）。4. 在无细胞系统中，新形成的磷脂酸转化为甘油酯的程度取决于酰基供体的性质、亚细胞颗粒的浓度、孵育时间和Mg(2+)的浓度。
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本文引用的文献
1
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2
Tissue fractionation studies. I. The existence of a mitochondria-linked, enzymically inactive form of acid phosphatase in rat-liver tissue.组织分级分离研究。I.大鼠肝脏组织中存在一种与线粒体相连的、酶活性失活形式的酸性磷酸酶。
Biochem J. 1951 Dec;50(2):174-81. doi: 10.1042/bj0500174.
3
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J Biol Chem. 1951 Feb;188(2):471-6.
4
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Fed Proc. 1961 Dec;20:934-40.
5
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6
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J Lipid Res. 1964 Oct;5:495-521.
7
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8
9
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