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1
A new assay procedure for monoglyceride acyltransferase.一种新的甘油单酯酰基转移酶检测方法。
Biochem J. 1974 Aug;141(2):407-11. doi: 10.1042/bj1410407.
2
The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate by the microsomal fraction of guinea-pig intestinal mucosa.豚鼠肠黏膜微粒体部分中棕榈酰辅酶A合成酶活性与sn-甘油3-磷酸酯化作用之间的关系。
Biochem J. 1973 Apr;132(4):707-15. doi: 10.1042/bj1320707.
3
Assay of acyl-CoA:monoglyceride acyltransferase from rat small intestine using continuous recording spectrophotometry.
J Lipid Res. 1969 Jul;10(4):427-32.
4
Triacylglycerol biosynthesis in human small intestinal mucosa. Acyl-CoA: monoglyceride acyltransferase.
Digestion. 1983;28(2):138-47. doi: 10.1159/000198977.
5
The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate in rat liver mitochondria.大鼠肝脏线粒体中棕榈酰辅酶A合成酶活性与sn-甘油3-磷酸酯化作用之间的关系
Biochem J. 1973 Apr;132(4):697-706. doi: 10.1042/bj1320697.
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Perinatal changes of the activity of acyl-CoA: monoglyceride acyltransferase in the rat intestinal mucosa.大鼠肠黏膜中酰基辅酶A：甘油单酯酰基转移酶活性的围产期变化
Experientia. 1973 Apr 15;29(4):405-7. doi: 10.1007/BF01926747.
7
Lack of dietary adaptation of intestinal 2-monoglyceride acyl transferase.肠道甘油单酯酰基转移酶缺乏饮食适应性。
Lipids. 1974 Nov;9(11):940-2. doi: 10.1007/BF02532624.
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Topographic distribution of enzymes involved in glycerolipid synthesis in rat small, intestinal epithelium.大鼠小肠上皮中甘油脂质合成相关酶的拓扑分布。
Biochim Biophys Acta. 1976 Dec 20;450(3):288-300. doi: 10.1016/0005-2760(76)90002-3.
9
Role of the surface excess of palmitoyl coenzyme A in the 1-acylglycerol-3-phosphate acyltransferase reaction catalyzed by microsomes.棕榈酰辅酶A表面过剩量在微粒体催化的1-酰基甘油-3-磷酸酰基转移酶反应中的作用。
J Biol Chem. 1975 Jun 25;250(12):4423-6.
10
Esterification of glycerol 3-phosphate in lactating guinea-pig mammary gland.豚鼠乳腺中3-磷酸甘油的酯化作用。
Biochem J. 1967 Oct;105(1):213-23. doi: 10.1042/bj1050213.

引用本文的文献

1
Enzymes of glycerolipid synthesis in small-intestinal mucosa of foetal and neonatal guinea pigs.胎儿和新生豚鼠小肠黏膜中甘油脂质合成的酶
Biochem J. 1975 Dec;152(3):675-9. doi: 10.1042/bj1520675.
2
The glycerol phosphate, dihydroxyacetone phosphate and monoacylglycerol pathways of glycerolipid synthesis in rat adipose-tissue homogenates.大鼠脂肪组织匀浆中甘油磷脂合成的磷酸甘油、磷酸二羟丙酮和单酰甘油途径。
Biochem J. 1976 Dec 15;160(3):693-700. doi: 10.1042/bj1600693.
3
The effect of chronic diabetes, induced by streptozotocin, on the activities of some enzymes of glycerolipid synthesis in rat liver.链脲佐菌素诱导的慢性糖尿病对大鼠肝脏甘油脂质合成中某些酶活性的影响。
Biochem J. 1977 Nov 15;168(2):147-53. doi: 10.1042/bj1680147.
4
Stearoyl-CoA desaturase activity in mammary adenocarcinomas carried by C3H mice.C3H小鼠所患乳腺腺癌中硬脂酰辅酶A去饱和酶的活性
Lipids. 1975 Dec;10(12):835-9. doi: 10.1007/BF02532329.

本文引用的文献

1
Chemical and physical properties of isomeric glyceryl monoethers.异构甘油单醚的化学和物理性质。
Lipids. 1967 Mar;2(2):161-71. doi: 10.1007/BF02530917.
2
Esterification of glycerol 3-phosphate in lactating guinea-pig mammary gland.豚鼠乳腺中3-磷酸甘油的酯化作用。
Biochem J. 1967 Oct;105(1):213-23. doi: 10.1042/bj1050213.
3
Purification and properties of triglyceride synthetase from the intestinal mucosa.肠黏膜甘油三酯合成酶的纯化及性质
Biochim Biophys Acta. 1966 Dec 7;125(3):465-73. doi: 10.1016/0005-2760(66)90035-x.
4
Assay of acyl-CoA:monoglyceride acyltransferase from rat small intestine using continuous recording spectrophotometry.
J Lipid Res. 1969 Jul;10(4):427-32.
5
Studies on the microsomal acylation of L-glycerol-3-phosphate. 3. Time course of the reaction.L-3-磷酸甘油的微粒体酰化作用研究。3. 反应的时间进程。
Biochim Biophys Acta. 1969 Jun 10;176(4):699-703. doi: 10.1016/0005-2760(69)90250-1.
6
The metabolism in vitro of enantiomeric I-O-alkyl glycerols and I,2-and I,3-alkyl acyl glycerols in the intestinal mucosa.
Biochim Biophys Acta. 1971 Jun 8;239(1):47-56. doi: 10.1016/0005-2760(71)90191-3.
7
The acylation of glycerophosphate in rat liver. A new assay procedure for glycerophosphate acylation, studies on its subcellular and submitochondrial localization and determination of the reaction products.
Biochim Biophys Acta. 1970 Jun 9;210(1):92-104. doi: 10.1016/0005-2760(70)90065-2.
8
The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate by the microsomal fraction of guinea-pig intestinal mucosa.豚鼠肠黏膜微粒体部分中棕榈酰辅酶A合成酶活性与sn-甘油3-磷酸酯化作用之间的关系。
Biochem J. 1973 Apr;132(4):707-15. doi: 10.1042/bj1320707.
9
The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate in rat liver mitochondria.大鼠肝脏线粒体中棕榈酰辅酶A合成酶活性与sn-甘油3-磷酸酯化作用之间的关系
Biochem J. 1973 Apr;132(4):697-706. doi: 10.1042/bj1320697.
10
A study of some enzymes of glycerolipid biosynthesis in rat liver after subtotal hepatectomy.大鼠肝大部切除术后甘油脂质生物合成的某些酶的研究。
Biochem J. 1973 May;134(1):103-12. doi: 10.1042/bj1340103.

一种新的甘油单酯酰基转移酶检测方法。

A new assay procedure for monoglyceride acyltransferase.

作者信息

Short V J, Brindley D N, Dils R

出版信息

Biochem J. 1974 Aug;141(2):407-11. doi: 10.1042/bj1410407.

DOI:10.1042/bj1410407
PMID:4455214
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168093/
Abstract
  1. A new assay system is described for monoglyceride acyltransferase (acylglycerol palmitoyltransferase, EC 2.3.1.22) in which palmitoyl-CoA is generated from palmitoyl-(-)-carnitine. 2. With the microsomal fraction from homogenates of guinea-pig intestinal mucosa, the V(max.) of this enzyme decreased with different acyl acceptors in the order 2-monopalmitoylglycerol>2-hexadecylglycerol>rac-1-monopalmitoylglycerol. 3. There were highly significant correlations between the monoglyceride acyltransferase activity as measured with these three substrates. This demonstrates that each of these substrates can be used to measure the same enzyme activity. 4. The advantages of using generated palmitoyl-CoA with 2-hexadecylglycerol and rac-1-monopalmitoylglycerol as model substrates for this enzyme are discussed.
摘要
  1. 描述了一种新的单甘油酯酰基转移酶（酰基甘油棕榈酰转移酶，EC 2.3.1.22）测定系统，其中棕榈酰辅酶A由棕榈酰-(-)-肉碱生成。2. 对于豚鼠肠黏膜匀浆的微粒体部分，该酶的V(max.)随不同酰基受体而降低，顺序为2-单棕榈酰甘油>2-十六烷基甘油>外消旋-1-单棕榈酰甘油。3. 用这三种底物测得的单甘油酯酰基转移酶活性之间存在高度显著的相关性。这表明这些底物中的每一种都可用于测量相同的酶活性。4. 讨论了使用生成的棕榈酰辅酶A与2-十六烷基甘油和外消旋-1-单棕榈酰甘油作为该酶的模型底物的优点。