一种新的甘油单酯酰基转移酶检测方法。
A new assay procedure for monoglyceride acyltransferase.
作者信息
Short V J, Brindley D N, Dils R
出版信息
Biochem J. 1974 Aug;141(2):407-11. doi: 10.1042/bj1410407.
Abstract
- A new assay system is described for monoglyceride acyltransferase (acylglycerol palmitoyltransferase, EC 2.3.1.22) in which palmitoyl-CoA is generated from palmitoyl-(-)-carnitine. 2. With the microsomal fraction from homogenates of guinea-pig intestinal mucosa, the V(max.) of this enzyme decreased with different acyl acceptors in the order 2-monopalmitoylglycerol>2-hexadecylglycerol>rac-1-monopalmitoylglycerol. 3. There were highly significant correlations between the monoglyceride acyltransferase activity as measured with these three substrates. This demonstrates that each of these substrates can be used to measure the same enzyme activity. 4. The advantages of using generated palmitoyl-CoA with 2-hexadecylglycerol and rac-1-monopalmitoylglycerol as model substrates for this enzyme are discussed.
摘要
- 描述了一种新的单甘油酯酰基转移酶（酰基甘油棕榈酰转移酶，EC 2.3.1.22）测定系统，其中棕榈酰辅酶A由棕榈酰-(-)-肉碱生成。2. 对于豚鼠肠黏膜匀浆的微粒体部分，该酶的V(max.)随不同酰基受体而降低，顺序为2-单棕榈酰甘油>2-十六烷基甘油>外消旋-1-单棕榈酰甘油。3. 用这三种底物测得的单甘油酯酰基转移酶活性之间存在高度显著的相关性。这表明这些底物中的每一种都可用于测量相同的酶活性。4. 讨论了使用生成的棕榈酰辅酶A与2-十六烷基甘油和外消旋-1-单棕榈酰甘油作为该酶的模型底物的优点。
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