Bonomo L, Dammacco F
Immunology. 1967 Dec;13(6):565-76.
The thyroglobulin antibodies present in the sera of thirteen leprosy patients were shown to be exclusively or predominantly macroglobulin in character when studied by diethylaminoethylcellulose chromatography and preparative ultracentrifugation. This was confirmed by immunoelectrophoresis and analytical ultracentrifugation of the fractions containing thyroglobulin antibodies, as well as by tests with 2-mercaptoethanol. The anti-thyroglobulin specificity of the antibodies showing macroglobulin character was ascertained by absorption experiments with human thyroglobulin; it was ruled out by absorption with FII that the 19S character might result from the formation of complexes with anti-γ-globulin factors. The conditions are discussed which may affect the 7S or 19S character of antibodies, in particular the antibody titre, the amount of antigenic stimulation and the vigour of the subject's immune response.
通过二乙氨基乙基纤维素色谱法和制备性超速离心法研究发现，13例麻风病人血清中的甲状腺球蛋白抗体在性质上完全或主要为巨球蛋白。通过对含有甲状腺球蛋白抗体的组分进行免疫电泳和分析超速离心，以及用2-巯基乙醇进行检测，证实了这一点。通过用人甲状腺球蛋白进行吸收实验，确定了具有巨球蛋白性质的抗体的抗甲状腺球蛋白特异性；通过用FII吸收排除了19S性质可能是由于与抗γ-球蛋白因子形成复合物所致。文中讨论了可能影响抗体7S或19S性质的条件，特别是抗体滴度、抗原刺激量和受试者免疫反应的强度。
