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豚草阿拉伯半乳聚糖的生物活性。I. 体外和体内效应的半乳糖特异性

The biological activity of a ragweed arabinogalactan. I. Galactose specificity of in vitro and in vivo effects.

作者信息

Kind L S, Nilsson B

出版信息

Immunology. 1967 Nov;13(5):477-82.

PMID:6073619
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1409183/
Abstract

A mouse antiserum to ragweed arabinogalactan (AGRg) agglutinated rabbit red blood cells coupled to AGRg by bis-diazotized benzidine. The antibodies to AGRg could be absorbed out with a yeast phosphogalactan but not with a yeast phosphomannan. A beet araban was also ineffective. Whole pollen grains from ragweed, timothy, wheat, barley and rye as well as commercial pollen extracts such as ragweed, timothy, sage and maple produced complete inhibition of haemagglutination. These studies suggest that cross-reactions between unrelated pollens previously reported may have been due to the presence of arabinogalactans in the materials employed. The permeability-enhancing effects of AGRg in mice could be completely inhibited by D-galactose, D-fucose or by galactose containing oligosaccharides. The stereochemical specificity of this inhibition suggests, but by no means proves, that the effects of AGRg are due to its interaction with natural antibody.

摘要

一种针对豚草阿拉伯半乳聚糖(AGRg)的小鼠抗血清能凝集通过重氮联苯胺偶联到AGRg的兔红细胞。针对AGRg的抗体可被酵母磷酸半乳聚糖吸收,但不能被酵母磷酸甘露聚糖吸收。甜菜阿拉伯聚糖也无作用。豚草、梯牧草、小麦、大麦和黑麦的全花粉粒以及豚草、梯牧草、鼠尾草和枫香等商业花粉提取物均能完全抑制血细胞凝集。这些研究表明,先前报道的不相关花粉之间的交叉反应可能是由于所用材料中存在阿拉伯半乳聚糖。AGRg对小鼠的通透性增强作用可被D-半乳糖、D-岩藻糖或含半乳糖的寡糖完全抑制。这种抑制作用的立体化学特异性表明,但绝不能证明,AGRg的作用是由于它与天然抗体的相互作用。

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Fatal anaphylaxis-like reaction induced by yeast mannans in nonsensitized mice.酵母甘露聚糖在未致敏小鼠中诱导的致死性类过敏反应。
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本文引用的文献

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PERMEABILITY FACTOR IN POLLEN EXTRACTS ACTIVE IN NON-SENSITIZED MICE.
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The effect of the sequence of erythrocyte sensitization on the bis-diazotized benzidine hemagglutination reaction.
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A factor in pollen which enhances vascular permeability.花粉中一种能增强血管通透性的因子。
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