Johnson U G, Porter K R
J Cell Biol. 1968 Aug;38(2):403-25. doi: 10.1083/jcb.38.2.403.
Cell division in log-phase cultures of the unicellular, biflagellate alga, Chlamydomonas reinhardi, has been studied with the electron microscope. The two basal bodies of the cell replicate prior to cytokinesis; stages in basal body formation are presented. At the time of cell division, the original basal bodies detach from the flagella, and the four basal bodies appear to be involved in the orientation of the plane of the cleavage furrow. Four sets of microtubules participate in cell division. Spindle microtubules are involved in a mitosis that is marked by the presence of an intact nuclear envelope. A band of microtubules arcs over the mitotic nucleus, indicating the future cleavage plane. A third set of microtubules appears between the daughter nuclei at telophase, and microtubules comprising the "cleavage apparatus" radiate from the basal bodies and extend along both sides of the cleavage furrow during cytokinesis. Features of cell division in C. reinhardi are discussed and related to cell division in other organisms. It is proposed that microtubules participate in the formation of the cleavage furrow in C. reinhardi.
利用电子显微镜对单细胞双鞭毛藻类莱茵衣藻对数期培养物中的细胞分裂进行了研究。细胞的两个基体在胞质分裂之前进行复制，文中展示了基体形成的各个阶段。在细胞分裂时，原来的基体从鞭毛上脱离，四个基体似乎参与了分裂沟平面的定向。四组微管参与细胞分裂。纺锤体微管参与有丝分裂，其特征是存在完整的核膜。一组微管在有丝分裂细胞核上方呈弧形，指示出未来的分裂平面。第三组微管在末期出现在子核之间，组成“分裂装置”的微管从基体放射状发出，并在胞质分裂期间沿着分裂沟的两侧延伸。文中讨论了莱茵衣藻细胞分裂的特征，并与其他生物体的细胞分裂进行了关联。有人提出微管参与了莱茵衣藻中分裂沟的形成。
