Vincent M M, Gitnick G L, Fabiyi A, Hensen S A, Sever J L, Huebner R J
Appl Microbiol. 1967 Sep;15(5):1198-201. doi: 10.1128/am.15.5.1198-1201.1967.
Rubella complement-fixing (CF) antigen and infectious virus were produced continuously and concurrently for as long as 63 days in suspension cultures of BHK-21 cells prepared from uncloned monolayer stock cultures. CF titers ranged from 1:4 to 1:32, and the peak infectivity titer was greater than 8.0 (TCID(50) log(10)) per ml. Suspension cultures could be recultivated after prolonged storage in liquid nitrogen. The resulting monolayer or suspension cultures also produced CF antigen. Suspension cultures provide an effective system for the long-term continuous and concurrent production of rubella virus diagnostic reagents.
风疹补体结合（CF）抗原和传染性病毒在由未克隆的单层原代培养物制备的BHK - 21细胞悬浮培养物中持续并同时产生长达63天。CF效价范围为1:4至1:32，每毫升的峰值感染性效价大于8.0（半数组织培养感染剂量（TCID50）对数（10））。悬浮培养物在液氮中长时间保存后可以再培养。由此产生的单层或悬浮培养物也能产生CF抗原。悬浮培养为风疹病毒诊断试剂的长期连续和同时生产提供了一个有效的系统。
