Gitnick G L, Vincent M M, Auletta A, Ley A, Tzan N, Sever J L
Appl Microbiol. 1967 Sep;15(5):1244-7. doi: 10.1128/am.15.5.1244-1247.1967.
Rubella virus specific hemagglutinating antigen was prepared in the fluid phase of suspension cultures. Systems employing baby hamster kidney culture adapted inocula, nonadapted inocula, and cells derived from long-term infected suspension cultures were evaluated. Optimal specific hemagglutinating titers were obtained when kaolin-treated fetal bovine serum was used in the media and when the incoulum had previously been adapted in a suspension culture system. When cultures derived from long-term infected suspension systems were studied, the number of daily harvests in which acceptable titers were present was prolonged. However, titers were generally higher in suspension systems employing cells which had recently been infected.
风疹病毒特异性血凝抗原是在悬浮培养的液相中制备的。对采用适应幼仓鼠肾细胞培养的接种物、未适应接种物以及来自长期感染悬浮培养物的细胞的系统进行了评估。当在培养基中使用高岭土处理的胎牛血清并且接种物先前已在悬浮培养系统中适应时，可获得最佳特异性血凝滴度。当研究来自长期感染悬浮系统的培养物时，出现可接受滴度的每日收获次数延长。然而，在采用最近感染细胞的悬浮系统中，滴度通常更高。
