Ioneda T, Giannotti O, Yoneda H
Arq Inst Biol (Sao Paulo). 1977 Jan-Jun;44(1-2):63-7.
A gas liquid chromatographic method for determination of catecholamines in small sample is described. The method involves homogenization of biological material into 0,1 M formic acid 5 mM ascorbic acid and subsequent conversion in their trimethylsilyl derivatives with BSA reagent and determined on 3% OV-1 column in a isothermal condition at 220 degrees C with the cromatograph equipped with hydrogen flame ionization. With respect to specificity, reproducibility and recovery, the results were satisfactory.
描述了一种用于测定小样本中儿茶酚胺的气相色谱方法。该方法包括将生物材料匀浆于0.1M甲酸 - 5mM抗坏血酸中，随后用BSA试剂将其转化为三甲基硅烷基衍生物，并在配备氢火焰离子化检测器的色谱仪上于220℃等温条件下在3% OV - 1柱上进行测定。在特异性、重现性和回收率方面，结果令人满意。
