Coombs J, Spanis C, Volcani B E
Plant Physiol. 1967 Nov;42(11):1607-11. doi: 10.1104/pp.42.11.1607.
Rates of photosynthesis, measured by oxygen electrode or by (14)CO(2) fixation, dark respiration and (32)P-phosphate incorporation are reported for the silicon-starvation synchrony of the fresh water diatom Navicula pelliculosa. During late exponential growth the rates were consistent with increase in carbon mass. During silicon starvation, rates of carbon dioxide fixation, oxygen evolution and (32)P incorporation fell, and the saturating light intensity decreased from 27,000 lux to 5000 lux. Reintroduction of silicon led to immediate transients in all parameters studied, followed by a prolonged increase in rate of dark respiration and a gradual increase in apparent photosynthesis. During release of daughter cells, the rates of dark respiration decreased as photosynthesis and (32)P incorporation increased. These results are discussed in relation to effects of silicon on the energy metabolism of the diatom.
报道了用氧电极或通过（14）CO₂固定、暗呼吸以及（32）P-磷酸盐掺入法测量的淡水硅藻纤细舟形藻硅饥饿同步过程中的光合作用速率。在指数生长后期，这些速率与碳质量的增加一致。在硅饥饿期间，二氧化碳固定、氧气释放和（32）P掺入速率下降，饱和光强从27000勒克斯降至5000勒克斯。重新添加硅导致所有研究参数立即出现瞬变，随后暗呼吸速率长时间增加，表观光合作用逐渐增加。在子细胞释放期间，暗呼吸速率下降，而光合作用和（32）P掺入增加。结合硅对硅藻能量代谢的影响对这些结果进行了讨论。
