Rasool C G, Selkoe D J
Brain Res. 1984 Nov 19;322(1):194-8. doi: 10.1016/0006-8993(84)91205-8.
In Alzheimer's disease, neurofibrillary tangles (NFT) react with both antibodies to paired helical filaments (PHF) and certain antibodies to neurofilaments, for example, monoclonal RT97 against the 200,000 mol. wt. neurofilament protein. After isolating NFT by extraction in sodium dodecyl sulfate (SDS), PHF antibodies remain reactive while RT97 weakly stains only a minority of the extracted tangles. We now show that isolated NFT initially unreactive with RT97 apparently develop exposed antigenic sites following incubation under denaturing conditions. Our results suggest that at least one neurofilament epitope is buried in the SDS-insoluble PHF fiber and that conformational changes in PHF proteins allow its exposures.
在阿尔茨海默病中，神经原纤维缠结（NFT）与针对双螺旋丝（PHF）的抗体以及某些针对神经丝的抗体发生反应，例如，针对200,000分子量神经丝蛋白的单克隆抗体RT97。在用十二烷基硫酸钠（SDS）提取法分离NFT后，PHF抗体仍具有反应性，而RT97仅对少数提取出的缠结物有微弱染色。我们现在表明，最初与RT97无反应性的分离出的NFT在变性条件下孵育后显然会出现暴露的抗原位点。我们的结果表明，至少一个神经丝表位被埋在SDS不溶性PHF纤维中，并且PHF蛋白的构象变化使其暴露出来。
