Characterization of monoclonal antibodies against acetylcholine receptors from Narke japonica.

Seven hybridoma clones were obtained which secrete monoclonal antibodies (mAbs) against nicotinic acetylcholine receptors from Japanese electric ray, Narke japonica. At least five, and probably seven, out of the seven mAbs recognized antigenic determinants located on the extramembranous surface of the receptor molecule. Binding competition between different mAbs to the receptor molecule was studied, and the results showed that these mAbs could be classified into three groups according to the distribution of their antigenic determinants.