Rabovsky J, Petersen M R, Lewis T R, Marion K J, Groseclose R D
J Toxicol Environ Health. 1984;14(5-6):655-66. doi: 10.1080/15287398409530615.
Male Fisher-344 rats were exposed by inhalation to low levels of diesel exhaust and coal dust, alone or in combination, or to filtered air, 7 h/d, 5 d/wk for 24 mo. Cytochrome P-450-associated benzo[a]pyrene hydroxylase and 7-ethoxycoumarin deethylase activities were assayed in lung and liver microsomes after 3, 6, and 24 mo. Age-related changes in enzyme activities were observed, but they were not altered by the exposures. When the data were adjusted for age, only one difference was observed. Lung benzo[a]pyrene hydroxylase activity in rats exposed to diesel exhaust and coal dust in combination was lower than that in animals exposed to coal dust alone (2.8 versus 4.4 pmol/min X mg protein). Neither value, however, differed significantly from the filtered-air controls, and no differences were observed in the other lung and liver activities. The data suggest exposure of the rats to diesel exhaust and/or coal dust had little or no effect on the selected lung and liver cytochrome P-450 activities under the conditions of the experiment.
将雄性Fisher-344大鼠每天7小时、每周5天暴露于低水平的柴油机废气和煤尘中，单独或联合暴露，或暴露于过滤空气中，持续24个月。在3个月、6个月和24个月后，对肺和肝微粒体中的细胞色素P-450相关的苯并[a]芘羟化酶和7-乙氧基香豆素脱乙基酶活性进行了测定。观察到酶活性随年龄的变化，但暴露并未改变这些变化。对数据进行年龄校正后，仅观察到一个差异。联合暴露于柴油机废气和煤尘的大鼠的肺苯并[a]芘羟化酶活性低于仅暴露于煤尘的动物（分别为2.8和4.4 pmol/分钟×毫克蛋白）。然而，这两个值与过滤空气对照组均无显著差异，在其他肺和肝活性方面也未观察到差异。数据表明，在实验条件下，大鼠暴露于柴油机废气和/或煤尘对所选的肺和肝细胞色素P-450活性几乎没有影响。
