N-甲基异靛红-β-4',4'-二乙硫代氨基脲对莫洛尼白血病病毒结构蛋白合成的抑制作用
Inhibition of the synthesis of Moloney leukemia virus structural proteins by N-methylisatin-beta-4',4'-diethylthiosemicarbazone.
作者信息
Ronen D, Teitz Y
出版信息
Antimicrob Agents Chemother. 1984 Dec;26(6):913-6. doi: 10.1128/AAC.26.6.913.
Abstract
The mechanism of inhibition of Moloney leukemia virus by N-methylisatin-beta-4',4'-diethylthiosemicarbazone was studied. Experiments that used [3H]leucine for short-pulse labeling in the presence of the drug resulted in a 71% inhibition in the synthesis of Pr-80, the polypeptide precursor of the gag viral proteins. The radioactive pulse products of the polypeptide precursors after a further 2-h chase period showed a normal cleavage of the precursors, with the formation of a reduced amount of final mature viral structural proteins. The experimental evidence indicated that at the inhibitory concentration of 17 microM N-methylisatin-beta-4',4'-diethylthiosemicarbazone, the amount of intracellular viral RNA was not affected, whereas the activities of reverse transcriptase and the other viral protein syntheses were suppressed.
摘要
研究了N-甲基异靛红-β-4',4'-二乙硫代半卡巴腙对莫洛尼白血病病毒的抑制机制。在药物存在的情况下，使用[3H]亮氨酸进行短脉冲标记的实验导致gag病毒蛋白的多肽前体Pr-80的合成受到71%的抑制。在进一步2小时的追踪期后，多肽前体的放射性脉冲产物显示前体正常裂解，最终成熟病毒结构蛋白的形成量减少。实验证据表明，在17 microM N-甲基异靛红-β-4',4'-二乙硫代半卡巴腙的抑制浓度下，细胞内病毒RNA的量不受影响，而逆转录酶的活性和其他病毒蛋白的合成受到抑制。
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