Salzberg S, Bakhanashvili M, Bari S, Berman I, Aboud M
J Virol. 1980 Sep;35(3):694-703. doi: 10.1128/JVI.35.3.694-703.1980.
We have recently found that Moloney murine leukemia virus assembles within cytoplasmic vacuoles of chronically infected NIH/3T3 cells rather than at their surface (submitted for publication). In the present study we found that if these cells were treated with interferon (IF) for 24 to 48 h the intracellular virus particles accumulated at a two- to threefold-higher level than that observed in untreated cells. Nevertheless, despite this accumulation, no difference between IF-treated and untreated cells was observed in the amount of the total cytoplasmic viral RNA or in its 35S or 21S species. When cellular virions were sedimented from the cytoplasmic fraction, a markedly higher amount of viral RNA was detected in the viral pellet of IF-treated cells than was detected in untreated cells, whereas the amount of viral RNA left in the virus-free cytoplasm of IF-treated cells was much lower than that in the untreated cells. Furthermore, the amount of the cytoplasmic polyriboadenylic acid-containing viral RNA was also remarkably higher in the IF-treated cells. Viral polyribosomes appeared to be fully functional in IF-treated cells, since no effect of IF on viral protein synthesis could be detected. Analysis of the nuclear viral RNA showed no difference between IF-treated and untreated cells after 24 h of IF treatment. Both contained a comparable amount of 35S viral RNA. However, at 48 h a significant accumulation of viral RNA was observed in the nucleus of the IF-treated cells as compared with the untreated cells, although in both cases only 35S species were evident. This accumulation appeared to activate a degradation process which destroyed nuclear viral RNA, since a dramatic shift toward smaller-sized molecules of viral RNA and a remarkable reduction in its amount were observed after 72 h of IF treatment.
我们最近发现，莫洛尼鼠白血病病毒在长期感染的NIH/3T3细胞的细胞质空泡内组装，而非在其表面组装（已提交发表）。在本研究中，我们发现，如果用干扰素（IF）处理这些细胞24至48小时，细胞内病毒颗粒的积累水平比未处理细胞中观察到的高两到三倍。然而，尽管有这种积累，在IF处理细胞和未处理细胞之间，总细胞质病毒RNA的量及其35S或21S种类均未观察到差异。当从细胞质部分沉淀细胞病毒粒子时，在IF处理细胞的病毒沉淀中检测到的病毒RNA量明显高于未处理细胞，而IF处理细胞的无病毒细胞质中剩余的病毒RNA量远低于未处理细胞。此外，IF处理细胞中含细胞质多聚腺苷酸的病毒RNA量也显著更高。病毒多核糖体在IF处理细胞中似乎功能完全正常，因为未检测到IF对病毒蛋白合成有影响。IF处理24小时后，对核病毒RNA的分析显示IF处理细胞和未处理细胞之间没有差异。两者都含有相当数量的35S病毒RNA。然而，在48小时时，与未处理细胞相比，在IF处理细胞的细胞核中观察到病毒RNA有显著积累，尽管在两种情况下只有35S种类明显可见。这种积累似乎激活了一个降解过程，该过程破坏了核病毒RNA，因为在IF处理72小时后，观察到病毒RNA向更小尺寸分子的显著转变及其量的显著减少。
