Ramirez de Guglielmone A E, Duvilanski B H
J Neurosci Res. 1977;3(1):11-20. doi: 10.1002/jnr.490030103.
A method to measure the "in vitro" RNA release from rat brain cell nuclei was described. Nuclear RNA was prelabelled "in vivo" for 30 or 120 min. In the first case the released RNA was heterogeneous and its electrophoretic mobility was similar to that of cytoplasmic messenger RNAs; nuclei prelabelled for 120 min mostly released the two major species of ribosomal RNAs. The release of mRNAs from the nuclei increased during cerebral development while that of the ribosomal RNAs did not. The increased capacity of the nuclei to release "radidly labelled" RNA with age neither determined an increase of the polysomal population, nor seemed to be dependent on cytoplasmic macromolecules.
描述了一种测量大鼠脑细胞核“体外”RNA释放的方法。核RNA在“体内”预先标记30分钟或120分钟。在第一种情况下，释放的RNA是异质的，其电泳迁移率与细胞质信使RNA相似；预先标记120分钟的核主要释放两种主要的核糖体RNA。在大脑发育过程中，细胞核中mRNA的释放增加，而核糖体RNA的释放则没有增加。随着年龄增长，细胞核快速释放“放射性标记”RNA的能力增加，这既没有导致多核糖体数量的增加，也似乎不依赖于细胞质大分子。
