Rigoni F, Panato L, Deana R
Int J Biochem. 1984;16(11):1121-5. doi: 10.1016/0020-711x(84)90004-1.
Phosphate efflux from uncoupled rat liver mitochondria was completely inhibited when mersalyl plus butylmalonate and ATP were added to a sucrose suspending medium. Despite the total retention of phosphate a calcium efflux was observed even in presence of ruthenium red. Under the above conditions no phosphate is transported in association with the ADP/ATP carrier. While mersalyl completely blocked the phosphate release induced by ruthenium red or EGTA from coupled mitochondria it only partially inhibited the CA2+-efflux. The inhibition of Ca2+ efflux was almost completely abolished in the presence of acetate. The existence of a co-transport of Ca2+ associated with phosphate is discussed.
当在蔗糖悬浮介质中加入汞撒利、丁基丙二酸酯和ATP时，解偶联大鼠肝线粒体的磷酸盐外流被完全抑制。尽管磷酸盐完全保留，但即使在钌红存在的情况下仍观察到钙外流。在上述条件下，没有磷酸盐与ADP/ATP载体一起转运。虽然汞撒利完全阻断了钌红或EGTA从偶联线粒体诱导的磷酸盐释放，但它仅部分抑制了Ca2+外流。在乙酸盐存在下，Ca2+外流的抑制几乎完全消除。讨论了与磷酸盐相关的Ca2+共转运的存在。
