Roos I, Crompton M, Carafoli E
Eur J Biochem. 1980 Sep;110(2):319-25. doi: 10.1111/j.1432-1033.1980.tb04870.x.
The effect of inorganic phosphate on Ca2+ retention has been investigated using phosphate-depleted liver mitchondria. Phosphate induces the release of Ca2+ through an efflux route insensitive to ruthenium red. This effect is not due to functional or structural damage, since mitochondria maintain their membrane potential during phosphate-induced Ca2+ efflux. Direct enzymatic measurement of mitochondria pyridine nucleotides has established that changes in their redox state (i.e. increased oxidation) do not play a role in the phosphate-effect. The phosphate-induced Ca2+ efflux requires transport of phosphate out of mitochondria. However, the fluxes of Ca2+ and phosphate do not coincide: the release of phosphate preceeds that of Ca2+.
利用缺磷的肝线粒体研究了无机磷酸盐对钙离子潴留的影响。磷酸盐通过对钌红不敏感的外流途径诱导钙离子释放。这种效应并非由于功能或结构损伤，因为线粒体在磷酸盐诱导的钙离子外流过程中保持其膜电位。对线粒体吡啶核苷酸的直接酶促测量表明，其氧化还原状态的变化（即氧化增加）在磷酸盐效应中不起作用。磷酸盐诱导的钙离子外流需要磷酸盐从线粒体中转运出来。然而，钙离子和磷酸盐的通量并不一致：磷酸盐的释放先于钙离子的释放。
