Shibuya A, Matsuo Y, Sagawa K, Katayama I
Hematol Oncol. 1984 Oct-Dec;2(4):359-64. doi: 10.1002/hon.2900020405.
Spiro and coworkers found a 35000 dalton membrane protein (p35) expressed abundantly in hairy cells of a subset of white, American patients with hairy cell leukemia. They subsequently identified p35 to be the human homologue of murine Ii, an electrophoretically invariant protein associated with Ia molecules. Using related analytical techniques, we have studied leukemic cells of two blood and one splenic samples from three Japanese patients with hairy cell leukemia and have demonstrated abundance of p35 in the splenic and one of the blood samples. The second blood sample was negative for p35 by our method of analysis, but the same hairy cells became positive for p35 at 2 months in culture and negative again at 4 months in culture. All 3 samples of this patient (fresh hairy cells and cultured cells at 2 and 4 months) remained consistently positive for Ia and for light chain-restricted immunoglobulin. Control cells from one each of acute lymphoblastic leukemia and Burkitt's lymphoma were negative for abundant p35 expression after culture as well as in the fresh state. One can conclude that the Japanese form of hairy cell leukemia, while varying in several distinct clinical and laboratory features from hairy cell leukemia in caucasians, is characterized also by abundant expression of p35 as seen in a subset of white patients. In addition, this expression of p35 is developed by hairy leukemic cells in culture initially but is lost upon longer term culture, in parallel with the level of tartrate-resistant acid phosphatase reactivity.
斯皮罗及其同事发现，在美国白人毛细胞白血病患者的一个亚组的毛细胞中大量表达一种35000道尔顿的膜蛋白（p35）。他们随后确定p35是小鼠Ii的人类同源物，Ii是一种与Ia分子相关的电泳不变蛋白。我们使用相关分析技术，研究了三名日本毛细胞白血病患者的两份血液样本和一份脾脏样本中的白血病细胞，发现脾脏样本和其中一份血液样本中p35含量丰富。通过我们的分析方法，第二份血液样本的p35呈阴性，但同样的毛细胞在培养2个月时p35呈阳性，在培养4个月时又转为阴性。该患者的所有3个样本（新鲜毛细胞以及培养2个月和4个月的细胞）的Ia和轻链限制性免疫球蛋白始终呈阳性。急性淋巴细胞白血病和伯基特淋巴瘤的对照细胞在培养后以及新鲜状态下p35均无大量表达。可以得出结论，日本型毛细胞白血病虽然在一些明显的临床和实验室特征方面与高加索人的毛细胞白血病有所不同，但也具有如部分白人患者那样p35大量表达的特征。此外，p35的这种表达最初由培养中的毛白血病细胞产生，但在长期培养后会消失，这与抗酒石酸酸性磷酸酶反应性水平一致。
