Spiro R C, Sairenji T, Humphreys R E
Leuk Res. 1984;8(1):55-62. doi: 10.1016/0145-2126(84)90031-6.
A molecule defining a subset of patients with hairy cell leukemia (HCL) on the basis of being abundantly labeled with [35S]methionine, was demonstrated to be the human homologue of murine Ii, a glycoprotein which lacks alloantigenic variation and is associated non-covalently with Ia antigens. In one-dimensional SDS-polyacrylamide gradient gel electrophoresis, the HCL-subset-defining molecule migrated with HLA-DR molecules which were immunoprecipitated with a specific heteroantiserum. These molecules were further defined in two-dimensional, SDS and non-equilibrium pH gradient electrophoresis of either membrane preparations or immunoprecipitates formed with various antibodies. [35S]methionine-labeling of the HCL-subset-defining molecule was greater in hairy leukemic cells than in lymphoblastoid cell lines. The subset-defining species was associated non-covalently with HLA-DR alpha and beta chains and ran electrophoretically at a position described for murine and human Ii molecules (in terms of pI and weight). Metabolic labeling of HLA-A,-B and -DR was also increased in HCL cells relative to lymphoblastoid cell lines. A separate protein, of 41,000 mol. wt and pI of 7-8, resembled another Ii-associated molecule which has been described in murine and human studies.
一种基于被[35S]甲硫氨酸大量标记来定义毛细胞白血病（HCL）患者亚群的分子，被证明是小鼠Ii的人类同源物，Ii是一种缺乏同种异型抗原变异且与Ia抗原非共价结合的糖蛋白。在一维SDS-聚丙烯酰胺梯度凝胶电泳中，定义HCL亚群的分子与用特异性异种抗血清免疫沉淀的HLA-DR分子一起迁移。这些分子在二维SDS和非平衡pH梯度电泳中进一步得到定义，电泳对象是膜制剂或用各种抗体形成的免疫沉淀物。毛细胞白血病细胞中定义HCL亚群的分子的[35S]甲硫氨酸标记比淋巴母细胞系中的更强。定义亚群的物种与HLA-DRα链和β链非共价结合，并在电泳时位于描述的小鼠和人类Ii分子的位置（就pI和分子量而言）。相对于淋巴母细胞系，HCL细胞中HLA-A、-B和-DR的代谢标记也增加。一种分子量为41,000且pI为7 - 8的单独蛋白质，类似于在小鼠和人类研究中描述的另一种与Ii相关的分子。
