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一种改进的差分脉冲伏安法技术可使用单个碳纤维电极在体内同时分析多巴胺能和5-羟色胺能活性。

An improved differential pulse voltammetry technique allows the simultaneous analysis of dopaminergic and serotonergic activities in vivo with a single carbon-fibre electrode.

作者信息

Crespi F, Paret J, Keane P E, Morre M

出版信息

Neurosci Lett. 1984 Nov 23;52(1-2):159-64. doi: 10.1016/0304-3940(84)90367-7.

DOI:10.1016/0304-3940(84)90367-7
PMID:6084835
Abstract

Differential pulse voltammetry has successfully been employed to study either 5-hydroxyindoles, or ascorbic acid and catechols in the brain of anaesthetised or freely moving rats. A new electrochemical pretreatment of pyrolytic carbon-fibre electrodes has been developed, enabling the simultaneous recording of all three compounds in the striatum of anaesthetised rats, using a Tacussel polarography. Furthermore, a fourth peak was recorded at +450 mV. Pharmacological treatments performed to define the nature of the four peaks recorded in the striatum confirmed that peak 1 corresponds to ascorbic acid, peak 2 to dihydroxyphenylacetic acid, peak 3 to 5-hydroxyindoleacetic acid and peak 4 to homovanillic acid.

摘要

差分脉冲伏安法已成功用于研究麻醉或自由活动大鼠大脑中的5-羟基吲哚、抗坏血酸和儿茶酚。已开发出一种新的热解碳纤维电极电化学预处理方法,使用塔库塞尔极谱法能够同时记录麻醉大鼠纹状体中的所有三种化合物。此外,在+450 mV处记录到第四个峰。为确定纹状体中记录的四个峰的性质而进行的药理治疗证实,峰1对应抗坏血酸,峰2对应二羟基苯乙酸,峰3对应5-羟基吲哚乙酸,峰4对应高香草酸。

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1
An improved differential pulse voltammetry technique allows the simultaneous analysis of dopaminergic and serotonergic activities in vivo with a single carbon-fibre electrode.一种改进的差分脉冲伏安法技术可使用单个碳纤维电极在体内同时分析多巴胺能和5-羟色胺能活性。
Neurosci Lett. 1984 Nov 23;52(1-2):159-64. doi: 10.1016/0304-3940(84)90367-7.
2
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[In vivo voltametric measurement of ascorbic acid and DOPAC in the striatum of the rat and guinea-pig].[大鼠和豚鼠纹状体中抗坏血酸和3,4-二羟基苯乙酸的活体伏安法测量]
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Semi-differential voltammetry with carbon fiber electrodes for in vivo determination of monoamine metabolites and ascorbic acid in rat corpus striatum.使用碳纤维电极的半微分伏安法用于体内测定大鼠纹状体中的单胺代谢物和抗坏血酸。
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Determination of dopamine, homovanillic acid and 3,4-dihydroxyphenylacetic acid in rat brain striatum by high-performance liquid chromatography with electrochemical detection.高效液相色谱-电化学检测法测定大鼠脑纹状体中的多巴胺、高香草酸和3,4-二羟基苯乙酸
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Functional in vivo interaction between growth hormone and dopamine systems are correlated to changes in striatal somatostatin levels as detected by voltammetry.通过伏安法检测发现,生长激素与多巴胺系统之间的功能性体内相互作用与纹状体生长抑素水平的变化相关。
Exp Brain Res. 1993;94(3):363-70. doi: 10.1007/BF00230196.
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In vivo evaluation by differential pulse voltammetry of the effect of thyrotropin-releasing hormone (TRH) on dopaminergic and serotoninergic synaptic activity in the striatum and nucleus accumbens of the rat.
通过差分脉冲伏安法对促甲状腺激素释放激素(TRH)对大鼠纹状体和伏隔核中多巴胺能和5-羟色胺能突触活性的影响进行体内评估。
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