Crespi F, Keane P E, Morre M
Br J Pharmacol. 1986 Jan;87(1):279-83. doi: 10.1111/j.1476-5381.1986.tb10181.x.
The effect of sodium valproate (VPA, 400 mg kg-1, i.p.) on extracellular ascorbate, 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) in the striatum was examined by differential pulse voltammetry in anaesthetized and freely-moving rats. In rats anaesthetized with chloral hydrate (400 mg kg-1, i.p.) pentobarbitone (50 mg kg-1, i.p.) or phenobarbitone (60 mg kg-1, i.p.), VPA produced no significant changes in peak 1 (extracellular ascorbate) or peak 2 (extracellular DOPAC), but produced a slight but statistically significant reduction in the height of peak 3 (extracellular 5-HIAA). In contrast, in freely-moving rats the same dose of VPA greatly reduced extracellular ascorbate and DOPAC concentrations, and increased that of 5-HIAA. These results suggest that VPA may reduce the release or turnover of dopamine, and increase that of 5-hydroxytryptamine in conscious rats. Our data also suggest that caution may be required in the interpretation of the effects of VPA in anaesthetized animals, as the results obtained may not always reflect the situation in the absence of anaesthesia.
通过差分脉冲伏安法，在麻醉和自由活动的大鼠中研究了丙戊酸钠（VPA，400 mg kg-1，腹腔注射）对纹状体细胞外抗坏血酸、3,4-二羟基苯乙酸（DOPAC）和5-羟吲哚乙酸（5-HIAA）的影响。在用氯水合醛（400 mg kg-1，腹腔注射）、戊巴比妥（50 mg kg-1，腹腔注射）或苯巴比妥（60 mg kg-1，腹腔注射）麻醉的大鼠中，VPA对峰1（细胞外抗坏血酸）或峰2（细胞外DOPAC）无显著影响，但使峰3（细胞外5-HIAA）的高度略有但具有统计学意义的降低。相比之下，在自由活动的大鼠中，相同剂量的VPA可显著降低细胞外抗坏血酸和DOPAC浓度，并增加5-HIAA的浓度。这些结果表明，VPA可能会减少清醒大鼠中多巴胺的释放或周转，并增加5-羟色胺的释放或周转。我们的数据还表明，在解释VPA对麻醉动物的影响时可能需要谨慎，因为所获得的结果可能并不总是反映无麻醉情况下的情况。
