Gattringer C, Wolf H, Wick G
Folia Biol (Praha). 1977;23(6):432-5.
Antigen-binding cells were enriched using bovine serum albumin immunized chickens as an experimental model. A comparison of density centrifugation, adherence to derivatized surfaces (polystyrol and gelatin) and affinity chromatography revealed that optimal enrichment was obtained using the latter procedure. Polyacrylamide was superior to agarose.
以牛血清白蛋白免疫鸡作为实验模型来富集抗原结合细胞。对密度离心、附着于衍生化表面(聚苯乙烯和明胶)及亲和层析进行比较后发现,采用后一种方法可实现最佳富集效果。聚丙烯酰胺优于琼脂糖。