携带蛋白质抗原（人血清白蛋白）受体的记忆细胞富集。
Enrichment of memory cells carrying receptors for a protein antigen (HSA).
作者信息
Sulitzeanu D, Axelrad M
出版信息
Immunology. 1973 May;24(5):803-11.
Sheep red cells (RC) were coupled with HSA by means of an HSA—anti-SRBC IgG conjugate. The coupled RC were used to prepare rosettes with spleen cells of mice immunized to HSA in Freund's complete adjuvant. The rosettes were fractionated on discontinuous Ficoll gradients and the rosette-rich and rosette-poor fractions were tested for ability to transfer adoptively secondary responsiveness to HSA. Efficiency of transfer was measured in terms of haemagglutination titres. Memory of HSA was shown to be associated with rosette-forming cells, i.e. with cells carrying receptors for HSA. Several parameters of the adoptive transfer system employed were studied. The HSA-coupled RC proved to be very efficient in stimulating secondary responses to HSA. High titres of haemagglutinating antibodies (up to 1/80,000) were obtained with 10 × 10 spleen cells transferred and 2 × 10 spleen cells consistently gave titres of the order of 1/10,000.
通过人血清白蛋白（HSA）-抗绵羊红细胞（SRBC）免疫球蛋白G（IgG）偶联物，将绵羊红细胞（RC）与人血清白蛋白偶联。用偶联的RC与在弗氏完全佐剂中免疫了人血清白蛋白的小鼠脾细胞制备玫瑰花结。将玫瑰花结在不连续的菲可梯度上进行分级分离，并检测富含玫瑰花结和贫玫瑰花结的级分过继转移对人血清白蛋白的二次反应性的能力。转移效率根据血凝滴度来衡量。已证明人血清白蛋白的记忆与形成玫瑰花结的细胞有关，即与携带人血清白蛋白受体的细胞有关。对所采用的过继转移系统的几个参数进行了研究。结果表明，与人血清白蛋白偶联的RC在刺激对人血清白蛋白的二次反应方面非常有效。转移10×10个脾细胞可获得高达1/80,000的高血凝抗体滴度，而转移2×10个脾细胞则始终产生约1/10,000的滴度。
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