Speiser F, Gottstein B
Acta Trop. 1984 Dec;41(4):361-72.
Two batches of excretory/secretory (E/S) antigens from second stage larvae of Toxocara canis maintained in vitro were prepared independently in two different laboratories (Zürich and Basel) and analysed in order to obtain information for future efforts to standardize the enzyme-linked immunosorbent assay (ELISA) used for the serodiagnosis of human toxocariasis. SDS-PAGE and "Western-blotting" revealed at least 10 different antigenic components common to the two antigen preparations. However, distinct qualitative and quantitative differences among the two E/S-antigens were observed, since one antigen had a more complex composition than the other. Despite these differences, an accordance of serodiagnosis was obtained in 80% of 25 sera from patients with suspected Toxocara infection tested independently in two different ELISA systems (Basel and Zürich) with the corresponding E/S-antigens. The specificity was 93% as determined (BS-antigen, BS-ELISA) by testing 46 out of 3396 sera from patients with parasitologically proven extra-intestinal helminthic infections. Cross-reactions occurred mainly with sera from patients infected with filariae (5 from 13 cases) exhibiting very high extinction values in their homologous ELISA-system. The reproducibility (intra- and inter-test variations) of two ELISA systems using the corresponding E/S-antigens varied from 5-15%. The results demonstrate that T. canis E/S-antigens may well be applicable for standardization of the ELISA used for the serodiagnosis of human toxocariasis.
在两个不同实验室（苏黎世和巴塞尔）分别独立制备了两批体外培养的犬弓首蛔虫二期幼虫的排泄/分泌（E/S）抗原，并进行分析，以便为今后标准化用于人类弓首蛔虫病血清诊断的酶联免疫吸附测定（ELISA）提供信息。SDS-PAGE和“Western印迹法”显示两种抗原制剂共有至少10种不同的抗原成分。然而，观察到两种E/S抗原在定性和定量方面存在明显差异，因为一种抗原的组成比另一种更复杂。尽管存在这些差异，但在使用相应E/S抗原的两种不同ELISA系统（巴塞尔和苏黎世）中对25份疑似弓首蛔虫感染患者的血清进行独立检测时，80%的血清诊断结果一致。通过对3396份经寄生虫学证实患有肠道外蠕虫感染患者的血清中的46份进行检测（BS抗原，BS-ELISA），确定特异性为93%。交叉反应主要发生在感染丝虫的患者血清中（13例中有5例），这些血清在其同源ELISA系统中呈现出非常高的吸光度值。使用相应E/S抗原的两种ELISA系统的重复性（试验内和试验间变异）在5%-15%之间。结果表明，犬弓首蛔虫E/S抗原很可能适用于标准化用于人类弓首蛔虫病血清诊断的ELISA。
