Manabe T, Takahashi Y, Okuyama T
Anal Biochem. 1984 Nov 15;143(1):39-45. doi: 10.1016/0003-2697(84)90555-4.
A new apparatus for electrophoretic transfer of proteins from micro polyacrylamide slab gels has been developed. The apparatus enabled the easy changing of nitrocellulose sheets and was suited for obtaining multiple blots from a gel. Electrophoretic conditions were determined so that all of the blots obtained sequentially from one slab gel were successfully used to visualize specific proteins irrespective of their molecular weight. Combining the transfer technique with the technique of parallel micro two-dimensional electrophoresis, 20 blots could be obtained within 1 h of electroblotting time. The locations of 28 human serum proteins were determined simultaneously on these blots using commercial specific antisera.
一种用于将蛋白质从微量聚丙烯酰胺平板凝胶中进行电泳转移的新装置已被开发出来。该装置能够轻松更换硝酸纤维素膜，并且适合从一块凝胶上获得多个印迹。确定了电泳条件，以便从一块平板凝胶上依次获得的所有印迹都能成功用于可视化特定蛋白质，而不论其分子量大小。将转移技术与平行微量二维电泳技术相结合，在1小时的电印迹时间内可获得20个印迹。使用市售的特异性抗血清在这些印迹上同时确定了28种人血清蛋白的位置。
