Miyata T, Isobe K, Dawson R, Ritter M A, Inagi R, Oda O, Taguchi R, Ikezawa H, Inoue I, Seo H
Department of Immunology, Nagoya University School of Medicine, Japan.
Immunology. 1990 Mar;69(3):391-5.
The molecular nature and cellular localization of Thy-1 antigen in human renal tissue were studied. Strong immunohistochemical staining was observed in frozen sections of human kidney using monoclonal anti-human Thy-1 antibody; this reaction was almost completely abolished by pretreating the kidney section with phosphatidyl inositol (PI)-specific phospholipase C (PI-PLC). Immunohistochemical analysis revealed that the Thy-1 antigen is localized on the proximal tubular epithelial cells and the Bowman's capsule of the glomerulus. Northern blot analysis of renal mRNA using a cloned human Thy-1 gene revealed the presence of human Thy-1 mRNA of a similar size to the one in human brain. When a human kidney cDNA library was screened with the same probe, a cDNA of human Thy-1 was isolated. Moreover, human Thy-1 protein with a molecular weight (MW) of 21,000 was detected in renal tissue by gel electrophoresis and Western blot analysis using monoclonal anti-human Thy-1 antibody. These data demonstrate for the first time the production of human Thy-1 as a PI-anchored protein with a unique cellular location in human renal tissue.
研究了人肾组织中Thy-1抗原的分子性质和细胞定位。使用单克隆抗人Thy-1抗体在人肾冰冻切片中观察到强免疫组织化学染色；用磷脂酰肌醇（PI）特异性磷脂酶C（PI-PLC）预处理肾切片后，该反应几乎完全消失。免疫组织化学分析显示，Thy-1抗原定位于近端肾小管上皮细胞和肾小球的鲍曼囊。使用克隆的人Thy-1基因对肾mRNA进行Northern印迹分析，结果显示存在与人脑中大小相似的人Thy-1 mRNA。当用相同探针筛选人肾cDNA文库时，分离出了人Thy-1的cDNA。此外，使用单克隆抗人Thy-1抗体通过凝胶电泳和Western印迹分析在肾组织中检测到分子量（MW）为21,000的人Thy-1蛋白。这些数据首次证明了人肾组织中产生了作为PI锚定蛋白且具有独特细胞定位的人Thy-1。
