鸟苷酸抗体：分级分离与特异性

Antibodies to guanylic acid: fractionation and specificities.

作者信息

Iswari S, Jacob T M

出版信息

Biosci Rep. 1984 Dec;4(12):1027-36. doi: 10.1007/BF01116695.

Abstract

Guanylic-acid-specific antibodies were elicited in rabbits, using as immunogen pG linked through 5'-phosphate to thyroglobulin. Specificity and affinity of antibodies to nucleotides, nucleosides, DNA, and RNA were studied by their binding to radioactive ligands and competition experiments. Guanylic-acid-specific antibodies do not bind to deoxyguanylic acid and have an average association constant of 10(7) M-1 at 4 degrees C. Binding of the antibodies to 3H-RNA is G-specific. The antibodies do not bind to 32P-ssDNA or 32P-dsDNA. The pG-specific antibodies could be separated into different fractions by affinity chromatography. These fractions, though specific to pG, differ in their cross-reactivities to nucleosides and nucleotides.

摘要

使用通过5'-磷酸与甲状腺球蛋白相连的pG作为免疫原，在兔体内诱导产生了鸟苷酸特异性抗体。通过抗体与放射性配体的结合及竞争实验，研究了其对核苷酸、核苷、DNA和RNA的特异性及亲和力。鸟苷酸特异性抗体不与脱氧鸟苷酸结合，在4℃时平均缔合常数为10⁷ M⁻¹。抗体与³H-RNA的结合具有G特异性。该抗体不与³²P-单链DNA或³²P-双链DNA结合。pG特异性抗体可通过亲和层析分离成不同组分。这些组分虽然对pG具有特异性，但对核苷和核苷酸的交叉反应性有所不同。