[抗单核苷酸抗体对体外RNA合成的抑制作用]
[Inhibition of RNA synthesis in vitro by antibodies against mononucleotides].
作者信息
Makarova O V, Gol'dfarb D M
出版信息
Mol Biol (Mosk). 1979 Nov-Dec;13(6):1413-9.
PMID:94937
Abstract
Antibodies against purine nucleotides were obtained from rabbits immunized with conjugates of bovine serum albumine with AMP or GMP. The antibodies purified by affinity chromatography on nucleoside monophosphate-human serum albumine-Sepharose columns inhibited RNA synthesis on native T4 phage DNA by E. coli RNA polymerase. The inhibition of transcription was due mainly to inhibition of the initiation stage of RNA synthesis.
摘要
通过用腺苷一磷酸（AMP）或鸟苷一磷酸（GMP）与牛血清白蛋白的偶联物免疫兔子，获得了抗嘌呤核苷酸抗体。通过在核苷单磷酸-人血清白蛋白-琼脂糖柱上进行亲和层析纯化的抗体，抑制了大肠杆菌RNA聚合酶对天然T4噬菌体DNA的RNA合成。转录的抑制主要是由于RNA合成起始阶段的抑制。
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