Thiebaut F, Rigaut J P, Feren K, Reith A
Biol Cell. 1984;52(2):103-8. doi: 10.1111/j.1768-322x.1985.tb00327.x.
Until recently scanning electron microscopes were mainly used to observe surfaces. However, it has been proved that a backscattered electron detector can give an image (BEI) of the specimen's internal structure after heavy metal staining. In this paper, we report how we have applied the silver staining for NOR-associated proteins to scanning electron microscopy, studying C3H10T1/2 cells in culture. This technique allows to localize, inside the nucleus, the nucleolar arrangement of AgNOR-associated proteins. In BEI imaging, the silver staining shows several intranucleolar silver spot-like deposits sometimes associated in "doublets" as on metaphasic chromosomes. These silver grains probably represent the fibrillar centre location, thought to be the interphasic counterpart of the NORs. However, these silver spot granules are more numerous during interphase.
直到最近，扫描电子显微镜主要用于观察表面。然而，已经证明，在重金属染色后，背散射电子探测器可以给出标本内部结构的图像（背散射电子图像）。在本文中，我们报告了我们如何将用于NOR相关蛋白的银染色应用于扫描电子显微镜，研究培养中的C3H10T1/2细胞。这项技术能够在细胞核内定位与AgNOR相关蛋白的核仁排列。在背散射电子图像成像中，银染色显示出几个核仁内的银点状沉积物，有时像在中期染色体上一样以“双联体”形式出现。这些银颗粒可能代表纤维中心的位置，被认为是NORs在间期的对应物。然而，这些银点颗粒在间期更多。
