Derenzini M, Farabegoli F, Trerè D
Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
Histochem J. 1992 Dec;24(12):951-6. doi: 10.1007/BF01046500.
We have studied the relationship between interphase nucleolar organizer region (NOR) distribution and nucleolar size in cancer cells at light-microscopical level. Thirteen cases of formalin-fixed bladder cancer and fifteen cases of methacarn-fixed tumours of different origin were used. Nucleoli of the former cases were stained by Phloxine B and of the latter by Toluidine Blue. Selective visualization of interphase NORs was obtained by carrying out the one-step silver staining reaction for AgNOR proteins (Ploton et al., 1986). The area occupied by Phloxine B- or Toluidine Blue-stained nucleoli and interphase silver-stained NORs was measured by means of an automated image analyser. Both in bladder cancers and in the other tumour lesions nucleolar and interphase AgNOR areas were linearly related (r = 0.95 and r = 0.96, respectively, P < 0.001). The close relationship between the area of nucleoli and that of silver-stained nucleolar structures was maintained even if the silver-staining procedure was prolonged beyond the optimal time length for selective interphase NOR staining. In the latter case, however, single interphase AgNORs were no longer visible within the nucleolar body which was, in fact, homogeneously stained. These data indicate that evaluation of the interphase AgNOR area has the same relevance, in tumour pathology, as whole nucleolar size measurement.
我们在光学显微镜水平上研究了癌细胞中分裂间期核仁组织区（NOR）分布与核仁大小之间的关系。使用了13例福尔马林固定的膀胱癌和15例不同来源的甲醇 Carnoy 固定肿瘤。前者的核仁用焰红B染色，后者用甲苯胺蓝染色。通过对AgNOR蛋白进行一步银染色反应（Ploton等人，1986年）实现分裂间期NOR的选择性可视化。用自动图像分析仪测量焰红B或甲苯胺蓝染色的核仁以及分裂间期银染色的NOR所占的面积。在膀胱癌和其他肿瘤病变中，核仁面积与分裂间期AgNOR面积均呈线性相关（分别为r = 0.95和r = 0.96，P < 0.001）。即使银染色程序延长至超过选择性分裂间期NOR染色的最佳时间长度，核仁面积与银染色核仁结构面积之间的密切关系仍然保持。然而，在后一种情况下，在实际上被均匀染色的核仁体内不再可见单个分裂间期AgNOR。这些数据表明，在肿瘤病理学中，评估分裂间期AgNOR面积与测量整个核仁大小具有相同的相关性。
