Robertson B H, Morgan D O, Moore D M
Virus Res. 1984 Sep;1(6):489-500. doi: 10.1016/0168-1702(84)90006-6.
The epitopes of six monoclonal antibodies generated against type A12 foot-and-mouth disease virus (FMDV) VP1 or its largest cyanogen bromide fragment (13 kd) were characterized. Five of these monoclonal antibodies neutralized viral infectivity. Solid-phase and competitive antigen binding assays using virion-derived antigens or a biosynthetic VP1 polypeptide identified two distinct neutralizing epitopes. One epitope was located between amino acid residues 145-168 of VP1 and the other between amino acids 169-179. The results indicate that antibodies reacting with two distinct areas of the VP1 polypeptide are capable of neutralizing FMD virus.
对针对A12型口蹄疫病毒（FMDV）VP1或其最大的溴化氰片段（13kd）产生的六种单克隆抗体的表位进行了鉴定。这些单克隆抗体中有五种可中和病毒感染性。使用病毒体衍生抗原或生物合成的VP1多肽进行的固相和竞争性抗原结合试验确定了两个不同的中和表位。一个表位位于VP1的氨基酸残基145 - 168之间，另一个位于氨基酸169 - 179之间。结果表明，与VP1多肽两个不同区域反应的抗体能够中和口蹄疫病毒。
