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口蹄疫病毒衣壳结构在异源系统中的表达、加工及组装：在豚鼠中诱导中和抗体反应

Expression, processing, and assembly of foot-and-mouth disease virus capsid structures in heterologous systems: induction of a neutralizing antibody response in guinea pigs.

作者信息

Lewis S A, Morgan D O, Grubman M J

机构信息

Plum Island Animal Disease Center, U.S. Department of Agriculture, Greenport, New York 11944.

出版信息

J Virol. 1991 Dec;65(12):6572-80. doi: 10.1128/JVI.65.12.6572-6580.1991.

DOI:10.1128/JVI.65.12.6572-6580.1991
PMID:1658362
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC250715/
Abstract

Plasmids containing the foot-and-mouth disease virus structural protein precursor (P1) and 3C protease genes or the P1 gene alone were expressed in Escherichia coli. A recombinant baculovirus containing the P1 gene was also generated and expressed in Spodoptera frugiperda cells. Expression of the P1 and 3C genes in E. coli resulted in efficient synthesis and processing of the structural protein precursor and assembly into 70S empty capsids. This material reacted with neutralizing monoclonal antibodies which recognize only conformational epitopes and elicited a significant neutralizing antibody response in vaccinated guinea pigs. Expression of the P1 gene in E. coli resulted in synthesis of an insoluble product, whereas in insect cells infected with the recombinant baculovirus a soluble product was synthesized. Both soluble and insoluble P1 reacted with a 12S-specific monoclonal antibody, but only soluble P1 elicited a neutralizing antibody response in guinea pigs.

摘要

含有口蹄疫病毒结构蛋白前体（P1）和3C蛋白酶基因或仅含P1基因的质粒在大肠杆菌中表达。还构建了含有P1基因的重组杆状病毒并在草地贪夜蛾细胞中表达。P1和3C基因在大肠杆菌中的表达导致结构蛋白前体的有效合成和加工，并组装成70S空衣壳。该物质与仅识别构象表位的中和单克隆抗体发生反应，并在接种疫苗的豚鼠中引发显著的中和抗体反应。P1基因在大肠杆菌中的表达产生了一种不溶性产物，而在感染重组杆状病毒的昆虫细胞中合成了一种可溶性产物。可溶性和不溶性P1均与12S特异性单克隆抗体发生反应，但只有可溶性P1在豚鼠中引发中和抗体反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/48a678714026/jvirol00055-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/3cb5d6edd2b4/jvirol00055-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/66fed6d077b8/jvirol00055-0211-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/949bac186b68/jvirol00055-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/b68b2c027ba9/jvirol00055-0212-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/48a678714026/jvirol00055-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/3cb5d6edd2b4/jvirol00055-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/66fed6d077b8/jvirol00055-0211-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/949bac186b68/jvirol00055-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/b68b2c027ba9/jvirol00055-0212-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c6/250715/48a678714026/jvirol00055-0213-a.jpg

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