Finter N B, Fantes K H, Johnston M
Dev Biol Stand. 1977;38:343-8.
Interferons have considerable antitumour effects in animals, and have been used with encouraging results in patients with osteocarcomas and certain other tumours. So far only relatively small amounts of material suitable for use in man have been prepared, and almost all of this has come from human white blood cells [buffy coats]. Human fibroblast cell lines are now increasingly being used as an alternative source, but the resultant interferon differs in its chemical and biological properties from leucocyte interferon. Lymphoblastoid cells can also be induced to form an interferon which appears identical to buffy coat interferon. These cells can be grown in suspension in large tanks, and could provide large amounts of relatively inexpensive interferon. The advantages of this type of production system and the problems associated with it will be discussed.
干扰素在动物体内具有显著的抗肿瘤作用，并且已用于治疗骨肉瘤和某些其他肿瘤患者，取得了令人鼓舞的效果。到目前为止，仅制备了相对少量适合人类使用的材料，而且几乎所有这些材料都来自人白细胞[血沉棕黄层]。人成纤维细胞系现在越来越多地被用作替代来源，但由此产生的干扰素在化学和生物学特性上与白细胞干扰素不同。淋巴母细胞也可被诱导形成一种与血沉棕黄层干扰素似乎相同的干扰素。这些细胞可以在大罐中悬浮培养，并能提供大量相对廉价的干扰素。将讨论这种生产系统的优点及其相关问题。
