人淋巴样干扰素的大规模生产与浓缩
Large-scale production and concentration of human lymphoid interferon.
作者信息
Klein F, Ricketts R T, Jones W I, DeArmon I A, Temple M J, Zoon K C, Bridgen P J
出版信息
Antimicrob Agents Chemother. 1979 Mar;15(3):420-7. doi: 10.1128/AAC.15.3.420.
Abstract
A stable and predictable production system is described for pilot plant quantities (milligram) of human lymphoid interferon, using suspension culture of an African Burkitt's lymphoma derived cell line Namalva with induction by Newcastle disease virus, B-1 strain. Cell cultures were grown in impeller-driven 50-liter fermentors with dilution of the postinduction culture using serum-free medium. High levels of dissolved oxygen were necessary for optimum cell growth. A total of 4,207 liters of interferon culture was produced in a series of 116 fermentor runs. An average yield of 3.5 log(10) international units of interferon per ml was realized before processing. Trichloroacetic acid was used to precipitate the interferon. An average of 3.35 log(10) international units of interferon per ml was recovered in the final nonpurified product.
摘要
描述了一种用于中试规模（毫克级）人淋巴干扰素生产的稳定且可预测的生产系统，该系统使用源自非洲伯基特淋巴瘤的Namalva细胞系进行悬浮培养，并由新城疫病毒B - 1株诱导。细胞培养物在叶轮驱动的50升发酵罐中生长，诱导后培养物用无血清培养基稀释。高水平的溶解氧对于最佳细胞生长是必要的。在一系列116次发酵罐运行中，共生产了4207升干扰素培养物。处理前每毫升干扰素的平均产量达到3.5 log(10)国际单位。使用三氯乙酸沉淀干扰素。最终未纯化产品中每毫升平均回收3.35 log(10)国际单位的干扰素。
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