Determination of immunoglobulin class and subclass of monoclonal antibodies to human alpha-fetoprotein by solid phase radioimmunoassay.

A solid phase radioimmunoassay using the incubation sequence: mouse immunoglobulin (Ig) rabbit anti-mouse Ig, monoclonal antibody and 125I-labeled antigen was performed to determine the class and subclass of seven murine monoclonal antibodies against human alpha-fetoprotein. All antibodies belonged to the IgG class. The subclass type was IgG 2a for four antibodies, IgG 1 for two antibodies and IgG 2b for one antibody.