Ryu E
Dev Biol Stand. 1977;38:381-5.
Of the 16 dyes studied, methylene blue med (MBM) was found to be the least and the most immunogenic either the dye-prepared vaccine or the dye solution injected simultaneously with ascites tumor (AT) cell suspension. The effective dosages of MBM ranged from 0.2 ml of 0.1% to 0.1 ml of 1%, i.e., 0.2-1.0 mg per mouse, weighed at 15-20 gm. Rabbits immunized with MBM-prepared vaccine produced specific antibody to AT in 2-3 weeks. Antisera produced by intraperitoneal injection were more effective than those produced by subcutaneous injection. Both rabbit immune serum and MBM dye solution were found to have therapeutic value in treatment of skin tumors induced by AT cell suspensions.
在所研究的16种染料中，发现亚甲蓝介质（MBM）无论是染料制备的疫苗还是与腹水瘤（AT）细胞悬液同时注射的染料溶液，其免疫原性都是最低且最高的。MBM的有效剂量范围为0.1%的0.2毫升至1%的0.1毫升，即每只体重15 - 20克的小鼠为0.2 - 1.0毫克。用MBM制备的疫苗免疫的兔子在2 - 3周内产生了针对AT的特异性抗体。腹腔注射产生的抗血清比皮下注射产生的抗血清更有效。发现兔免疫血清和MBM染料溶液在治疗由AT细胞悬液诱导的皮肤肿瘤方面都具有治疗价值。
