A vaccine and immune serum for ascites tumor of mice and therapy of skin tumors.

Of the 16 dyes studied, methylene blue med (MBM) was found to be the least and the most immunogenic either the dye-prepared vaccine or the dye solution injected simultaneously with ascites tumor (AT) cell suspension. The effective dosages of MBM ranged from 0.2 ml of 0.1% to 0.1 ml of 1%, i.e., 0.2-1.0 mg per mouse, weighed at 15-20 gm. Rabbits immunized with MBM-prepared vaccine produced specific antibody to AT in 2-3 weeks. Antisera produced by intraperitoneal injection were more effective than those produced by subcutaneous injection. Both rabbit immune serum and MBM dye solution were found to have therapeutic value in treatment of skin tumors induced by AT cell suspensions.