Madrzak C J, Konieczny A, Legocki A B
Acta Biochim Pol. 1984;31(4):439-51.
Complementary DNA (cDNA) synthesis was performed using mRNA from lupin root nodules and from lupin uninfected roots as templates. Optimal conditions for the synthesis of 700 nucleotides long cDNA were established. Hybridization kinetics of mRNA-cDNA were performed in a homologous system from root nodules as well as in a heterologous system. Hybridization analysis revealed the presence of four frequency abundance classes within the root nodule mRNA population. In order to enrich the cDNA population in leghaemoglobin sequences, two synthetic pentadecanucleotide fragments complementary to the putative 3' ends of leghaemoglobin mRNAs were used as primers for the reverse transcription of nodule poly(A)-RNA. The resultant cDNA was enriched in rapidly hybridizing components which may contain leghaemoglobin sequences.
以羽扇豆根瘤和未感染根的mRNA为模板进行互补DNA（cDNA）合成。确定了合成700个核苷酸长cDNA的最佳条件。在来自根瘤的同源系统以及异源系统中进行了mRNA-cDNA的杂交动力学研究。杂交分析揭示了根瘤mRNA群体中存在四个频率丰度类别。为了使cDNA群体中富含豆血红蛋白序列，将两个与豆血红蛋白mRNA假定3'端互补的合成十五核苷酸片段用作根瘤多聚腺苷酸RNA逆转录的引物。所得的cDNA富含可能包含豆血红蛋白序列的快速杂交成分。
