Konieczny A, Szczygłowski K, Madrzak C J, Legocki A B
Acta Biochim Pol. 1987;34(1):21-7.
Yellow lupin nodule specific sequences were selected by screening of cDNA library prepared from lupin nodule poly(A)+RNA. From about 3,000 clones containing fragments of lupin DNA 150-1,500 base pair long, 7% of clones carrying nodule specific sequences were identified. Among them the most abundant sequence species, represented by 32% clones, encodes leghemoglobin. Another abundant species designated pLN13 is represented by 13% clones. The Northern blot analysis of lupin mRNA confirmed nodule specificity of the cloned sequences. The nucleotide sequence of one clone, pLN281 of 225 bp, is presented.
通过筛选从羽扇豆根瘤多聚腺苷酸加尾RNA制备的cDNA文库，选择了羽扇豆根瘤特异性序列。从大约3000个含有长度为150 - 1500碱基对的羽扇豆DNA片段的克隆中，鉴定出7%携带根瘤特异性序列的克隆。其中，以32%的克隆为代表的最丰富的序列种类编码豆血红蛋白。另一种丰富的种类称为pLN13，以13%的克隆为代表。羽扇豆mRNA的Northern印迹分析证实了克隆序列的根瘤特异性。给出了一个225 bp的克隆pLN281的核苷酸序列。
