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鼠伤寒沙门氏菌包膜突变体的生化及表型校正

Biochemical and phenotypical correction of an envelope mutant of Salmonella typhimurium.

作者信息

Pizarro R A, Boselli G O, Orce L V

出版信息

Arch Int Physiol Biochim. 1984 Dec;92(5):333-7. doi: 10.3109/13813458409080608.

DOI:10.3109/13813458409080608
PMID:6085548
Abstract

Salmonella typhimurium DA 361 bears an env D1 mutation with the following abnormal phenotypical and biochemical characteristics: a) it autolyses at stationary phase in nutrient broth; b) it grows in chains of short rods; c) it is a poor maltose fermenter and d) it has a diphosphatidylglycerol (DPG) content twice as high than its isogenic non-lytic pair DA 362 (env D+) and LT2, of which both are derivatives. Growth of DA 361 in the presence of 400 mM ethanol leads on a 50% decrease of DPG level, thereby equalling its PG/DPG ratio with those of the control strain. Consequently, a correction on the other phenotypical and biochemical anomalies are induced since the DA 361 strain decreases its autolytic activity, ferments normally maltose and appear as rods undifferentiated from DA 362.

摘要

鼠伤寒沙门氏菌DA 361带有env D1突变,具有以下异常的表型和生化特征:a) 它在营养肉汤的稳定期自溶;b) 它以短杆菌链的形式生长;c) 它是一种不良的麦芽糖发酵菌;d) 它的二磷脂酰甘油(DPG)含量是其同基因非裂解菌株DA 362(env D+)和LT2(两者均为衍生物)的两倍。在400 mM乙醇存在的情况下,DA 361的生长导致DPG水平降低50%,从而使其PG/DPG比值与对照菌株相等。因此,由于DA 361菌株降低了其自溶活性、正常发酵麦芽糖并呈现出与DA 362无差异的杆菌形态,其他表型和生化异常也得到了纠正。

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Linkage map of Salmonella typhimurium, edition VII.鼠伤寒沙门氏菌连锁图谱,第七版。
Microbiol Rev. 1988 Dec;52(4):485-532. doi: 10.1128/mr.52.4.485-532.1988.